PMS1 antibody

Catalog No. ABIN7126799

This Mouse Monoclonal antibody specifically detects PMS1 in ELISA, IP, FACS, IF and ISt. It exhibits reactivity toward Human.

Quick Overview for PMS1 antibody (ABIN7126799)

Target: PMS1 (PMS1 Postmeiotic Segregation Increased 1 (S. Cerevisiae) (PMS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PMS1 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Product Details anti-PMS1 Antibody

Specificity: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45 % of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Two additional homologs of the prokaryotic MutL gene, designated PMS1 and PMS2, have been identified and shown to be mutated in the germline of HNPCC patients.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PMS1 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (concentration is assay dependent), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U-251 MG or CACO-2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PMS1

Target: PMS1 (PMS1 Postmeiotic Segregation Increased 1 (S. Cerevisiae) (PMS1))

Alternative Name: PMS1

Background: DNA mismatch repair protein PMS1, HNPCC3, hPMS1, Human homolog of yeast mutL, PMS1 postmeiotic segregation increased 1 (S. cerevisiae), PMS1 protein homolog 1, PMSL1, Rhabdomyosarcoma antigen MU RMS 40.10B / 40.10E,PMS1
Cellular localisation: Nucleus. Nuclear bodies.

Molecular Weight: 105.8kDa

Gene ID: 5378, 111749

UniProt: P54277