Recombinant PMS2 antibody (AA 1-100)

Catalog No. ABIN7126800

The Rabbit Monoclonal anti-PMS2 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect PMS2 in samples from Human.

Quick Overview for Recombinant PMS2 antibody (AA 1-100) (ABIN7126800)

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PMS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-PMS2 Antibody

Binding Specificity: AA 1-100

Specificity: PMS2 is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15 % of all colon cancers. Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS), also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide corresponding to residues within aa1-100 of human PMS2 (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PMS2

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Alternative Name: PMS2

Background: DNA mismatch repair protein PMS2, hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Mismatch repair gene PMSL2, PMS1 protein homolog 2, postmeiotic segregation increased 2 (S. cerevisiae), PMS2CL,PMS2 (Postmeiotic Segregation Increased 2)
Cellular localisation: Nuclear

Molecular Weight: 96kDa

Gene ID: 5395, 632637

UniProt: P54278

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response