PRMT7 antibody

Catalog No. ABIN7126811

This Mouse Monoclonal antibody specifically detects PRMT7 in WB, IF, IP and FACS. It exhibits reactivity toward Human.

Quick Overview for PRMT7 antibody (ABIN7126811)

Target: PRMT7 (Protein Arginine Methyltransferase 7 (PRMT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRMT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-PRMT7 Antibody

Specificity: Arginine methylation is an irreversible protein modification catalyzed by Arginine methyltransferases, such as PRMT7, which uses S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport and RNA splicing. PRMT7 has two methyltransferase domains, each containing a putative AdoMet-binding motif. The N-terminal methyltransferase domain closely resembles the catalytic core of PRMT5, and the C-terminal domain is most similar to that of PRMT1. Three PRMT7 splice variants have been identified by database analysis. PRMT7 is localized to the nucleus and cytoplasm and moderate expression is observed in adult brain and lung tissues.

Cross-Reactivity (Details): Human. Predicted to react with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PRMT7 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Western Blot (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PRMT7

Target: PRMT7 (Protein Arginine Methyltransferase 7 (PRMT7))

Alternative Name: PRMT7

Background: [Myelin basic protein]-arginine N-methyltransferase PRMT7, Histone-arginine N-methyltransferase PRMT7, KIAA1933, Protein arginine methyltransferase 7, Protein arginine N-methyltransferase 7,PRMT7
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molecular Weight: 78.46kDa

Gene ID: 54496, 640229

UniProt: Q9NVM4

Pathways: Ribonucleoprotein Complex Subunit Organization