Recombinant ATRX antibody

Catalog No. ABIN7126814

This Mouse Monoclonal antibody specifically detects ATRX in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant ATRX antibody (ABIN7126814)

Target: ATRX (helicase 2, X-linked (ATRX))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATRX antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RATRX-3446

Product Details anti-ATRX Antibody

Purpose: Recombinant ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Antibody

Specificity: ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 °™ to mediate chromosomal segregation. Mutations in the ATRX gene correlate with a high incidence of severe X-linked form of syndromal mental retardation associated with °™ thalassemia or ATRX syndrome

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ATRX protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji, HeLa or A431 cells. Pancreas, Adrenal, Ovary, Kidney, Prostate or Stomach.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX

Background:

Alpha thalassemia/mental retardation syndrome X linked homolog, ATP-dependent helicase ATRX, ATR2, ATRX, DNA-dependent ATPase and helicase, MRXHF1, RAD54, RAD54L, SFM1, SHS, Transcriptional regulator ATRX, X-linked helicase II, X-linked nuclear protein, XH2, XNP, Znf-HX

adrenal, HeLa or A431 cells. Human pancreas, Kidney, ovary, prostate or stomach., Raji

Molecular Weight: 280kDa

Gene ID: 546, 533526, 653797

UniProt: P46100