OCT4 antibody (AA 1-134)

Catalog No. ABIN7126815

The Mouse Monoclonal anti-OCT4 antibody is suitable to detect OCT4 in samples from Human. It has been validated for WB and IHC.

Quick Overview for OCT4 antibody (AA 1-134) (ABIN7126815)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: Oct4-3508

Product Details anti-OCT4 Antibody

Binding Specificity: AA 1-134

Purpose: OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody

Specificity: OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells, its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Cross-Reactivity (Details): Human

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji or U-937 cells. Human seminoma or testicular carcinoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background:

OCT3, OCT3A, OCT4, OCT4A, OCT-3/4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, octamer-binding transcription factor-3, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1

Raji or U-937 cells. Human seminoma or testicular carcinoma. NCCIT.

Molecular Weight: 38.6kDa

Gene ID: 5460, 249184

UniProt: Q01860

Pathways: Stem Cell Maintenance