PSMD4/ASF antibody (Regulatory Subunit 4)

Catalog No. ABIN7126850

This Mouse Monoclonal antibody specifically detects PSMD4/ASF in WB. It exhibits reactivity toward Human, Rat, Mouse, Rabbit, Guinea Pig and Hamster.

Quick Overview for PSMD4/ASF antibody (Regulatory Subunit 4) (ABIN7126850)

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Reactivity: Human, Rat, Mouse, Rabbit, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMD4/ASF antibody is un-conjugated

Application: Western Blotting (WB)

Clone: CPTC-PSMD4-3

Product Details anti-PSMD4/ASF Antibody

Binding Specificity: Regulatory Subunit 4

Purpose: 26S proteasome non-ATPase regulatory subunit 4 Antibody

Specificity: In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human PSMD4protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or HepG2 cells. Liver tissue lysate. Human liver, tonsil or kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PSMD4/ASF

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Alternative Name: PSMD4

Background:

26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, AF, AF-1

Jurkat or HepG2 cells. Liver tissue lysate. Human liver, Tonsil or Kidney. HeLa, K-562, Human Skeletal Muscle.

Molecular Weight: ~50kDa

Gene ID: 5710, 505059

UniProt: P55036

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway