RPA2 antibody

Catalog No. ABIN7126904

This Mouse Monoclonal antibody specifically detects RPA2 in WB, IHC and IHC (f). It exhibits reactivity toward Human.

Quick Overview for RPA2 antibody (ABIN7126904)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-RPA2 Antibody

Specificity: Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length Replication Protein A2 (RPA2) protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa or HepG2 cells. Human tonsil (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2

Background: P32, p34, REPA2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, Replication protein A2, RF-A protein 2, Rf-A2, RFA, RP-A p32, RP-A p34, RP21C, RPA32, RPA34, RpLP1, RpP2,Replication Protein A2 (RPA2)
Cellular localisation: Nuclear

Molecular Weight: 32-34kDa

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA