Recombinant RRM1 antibody (AA 700-800)

Catalog No. ABIN7126906

The Rabbit Monoclonal anti-RRM1 antibody (Clone RRM1-4372R) (ABIN7126906) specifically detects RRM1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant RRM1 antibody (AA 700-800) (ABIN7126906)

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RRM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RRM1-4372R

Product Details anti-RRM1 Antibody

Binding Specificity: AA 700-800

Purpose: Recombinant Ribonucleotide Reductase M1 / RRM1 Antibody

Specificity: Ribonucleotide reductase M1 polypeptide (RRM1) is one of two non-identical subunits for ribonucleoside-diphosphate reductase, an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. RRM1 is present throughout the cell division cycle but downregulated in quiescent cells. RRM1 is involved in carcinogenesis, tumor progression, and the response of non-small-cell lung cancer (NSCLC) to chemotherapy.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa700-800 of human RRM1 (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human breast, lung or colon carcinoma tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Alternative Name: RRM1

Background:

Ribonucleoside diphosphate reductase large subunit, Ribonucleoside diphosphate reductase M1 chain, RR1, RRM1, RIR1, Ribonucleotide reductase M1 polypeptide, Ribonucleotide reductase R1 subunit

Human breast, lung or colon carcinoma tissue. Human Intestine, Human Skin, A549, HEK293 or K-562

Molecular Weight: 94kDa

Gene ID: 6240, 445705

Pathways: Positive Regulation of Endopeptidase Activity