SET/TAF-I antibody

Catalog No. ABIN7126932

Product Details anti-SET/TAF-I Antibody

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: Protein phosphatase 2A (PP2A) is a major mammalian protein serine/threonine phosphatase that regulates diverse cellular processes. Inhibitor 1 of PP2A (I1PP2A) and inhibitor 2 of PP2A (I2PP2A), which share large sequence similarity, are heat-stable protein inhibitors of the cellular phosphatase activity of PP2A. I1PP2A and I2PP2A were initially characterized as putative HLA class II associated proteins Phap I and Phap II. These inhibitor proteins act noncompetitively to selectively inhibit PP2A, but do not affect the phosphatase activity of the related proteins PP1, PP2B and PP2C. The I1PP2A protein is localized to both the cytoplasm and the nucleus. In contrast, I2PP2A is located predominantly in the nucleus and is highly expressed in Wilms tumor cells. Transient expression of I2PP2A in HEK-293 cells leads to an increase in the DNA binding activity of the proto-oncogene c-Jun.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human SET protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET (SET Products)

Synonyms: 2PP2A antibody, I2PP2A antibody, IGAAD antibody, IPP2A2 antibody, PHAPII antibody, TAF-I antibody, TAF-IBETA antibody, 2610030F17Rik antibody, 5730420M11Rik antibody, AA407739 antibody, I-2PP2A antibody, StF-IT-1 antibody, 2pp2a antibody, i2pp2a antibody, igaad antibody, ipp2a2 antibody, phapii antibody, set antibody, set-a antibody, set-b antibody, taf-ibeta antibody, wu:fb30g11 antibody, wu:fd16c08 antibody, SET nuclear proto-oncogene antibody, SET nuclear oncogene antibody, SET translocation antibody, SET nuclear proto-oncogene L homeolog antibody, SET nuclear proto-oncogene b antibody, SET antibody, Set antibody, LOC403555 antibody, set.L antibody, setb antibody

Background: 2PP2A, HLA DR associated protein II, IGAAD, Inhibitor of granzyme A activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPII, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, TAFI, Template-Activating Factor-I, chromatin remodelling factor, SET nuclear oncogene, SET translocation (myeloid leukemia-associated),SET
Cellular localisation: Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm.

Molecular Weight: 39kDa

Gene ID: 6418, 436687

UniProt: Q01105

Pathways: Apoptosis