SMARCC1 antibody

Catalog No. ABIN7126945

The Mouse Monoclonal anti-SMARCC1 antibody is suitable to detect SMARCC1 in samples from Human. It has been validated for IF and FACS.

Quick Overview for SMARCC1 antibody (ABIN7126945)

Target: SMARCC1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily C, Member 1 (SMARCC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCC1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SMARCC1 Antibody

Specificity: The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2i±) and Brg-1 (also designated SNF2 or SNF2i²) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human SMARCC1 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, K562 or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SMARCC1

Target: SMARCC1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily C, Member 1 (SMARCC1))

Alternative Name: SMARCC1

Background: AI115498, BRG1-associated factor 155 (BAF155), Chromatin remodeling complex BAF155 subunit (CRACC1), Mammalian chromatin remodeling complex BRG1 associated factor 155, Rsc8, SRG3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1,SMARCC1 / BAF155
Cellular localisation: Nucleus.

Molecular Weight: 123kDa

Gene ID: 6599, 476179

UniProt: Q92922

Pathways: Chromatin Binding