SOX4 antibody (AA 45-130)

Catalog No. ABIN7126957

The Mouse Monoclonal anti-SOX4 antibody is suitable to detect SOX4 in samples from Human. It has been validated for WB, IHC, IF and FACS.

Quick Overview for SOX4 antibody (AA 45-130) (ABIN7126957)

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SOX4-1D6

Product Details anti-SOX4 Antibody

Binding Specificity: AA 45-130

Purpose: SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) Antibody

Specificity: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF- æ-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival invitro and for primary tumor growth and metastasis invivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.

Cross-Reactivity (Details): Human. Predicted to react in Xenopus and Chick.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa45-130) of human SOX4 protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Colon or breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX4

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Alternative Name: SOX4

Background:

SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4

HeLa cells. Colon or breast carcinoma.

Molecular Weight: 47kDa

Gene ID: 6659, 643910

UniProt: Q06945