SOX9 antibody (AA 393-508)

Catalog No. ABIN7126960

The Mouse Monoclonal anti-SOX9 antibody has been validated for IHC (f). It is suitable to detect SOX9 in samples from Human.

Quick Overview for SOX9 antibody (AA 393-508) (ABIN7126960)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SOX9 Antibody

Binding Specificity: AA 393-508

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTMArray, containing more than 19,000, full-length human proteins.Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant humanSOX9 protein fragment (aa 393-508) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells. Human pancreas

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 / SRY-box 9
Cellular localisation: Nuclear

Molecular Weight: 65kDa

Gene ID: 6662, 647409

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development