SOX10 antibody (AA 90-179)

Catalog No. ABIN7126962

This Mouse Monoclonal antibody specifically detects SOX10 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for SOX10 antibody (AA 90-179) (ABIN7126962)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SOX10 Antibody

Binding Specificity: AA 90-179

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes & myoepithelial cells of salivary, bronchial & mammary glands. SOX-10 expression is also observed in mast cells.

Cross-Reactivity (Details): Human. Predicted to work with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human SOX10 protein fragment (around aa90-179) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: COLO-38 or HepG2 cells. Human melanomas, breast carcinomas or gliomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C),SOX10 (Melanoma Marker)
Cellular localisation: Nuclear

Molecular Weight: 49-58kDa

Gene ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding