SPTAN1 antibody (AA 2351-2475)

Catalog No. ABIN7126980

This Mouse Monoclonal antibody specifically detects SPTAN1 in IHC and IHC (f). It exhibits reactivity toward Human.

Quick Overview for SPTAN1 antibody (AA 2351-2475) (ABIN7126980)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTAN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SPTAN1 Antibody

Binding Specificity: AA 2351-2475

Specificity: Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous i± and i² chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin i± I and spectrin i² I are present in erythrocytes, whereas spectrin i± II (also designated fodrin i±) and spectrin i² II (also designated fodrin i²) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin i± II, a neuronal cytoskeleton protein.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human SPTAN1 protein (around aa2351-2475) (exact sequence is proprietary))

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human colon or kidney tissues (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: SPTAN1

Background: Alpha-II spectrin, brain, EIEE5, Fodrin alpha chain, NEAS, Non erythrocytic spectrin alpha, non-erythroid alpha chain, SPECA, Spectrin alpha chain brain, Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spna2, SPTA2,Fodrin / Alpha Spectrin II (SPTAN1) / NEAS
Cellular localisation: Cytoplasm

Molecular Weight: 240kDa

Gene ID: 6709, 372331

UniProt: Q13813

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization