Recombinant Synaptophysin antibody (AA 224-313)

Catalog No. ABIN7126991

This anti-Synaptophysin antibody is a Mouse Monoclonal antibody detecting Synaptophysin in IHC (f). Suitable for Human.

Quick Overview for Recombinant Synaptophysin antibody (AA 224-313) (ABIN7126991)

Target: Synaptophysin (SYP)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Synaptophysin Antibody

Binding Specificity: AA 224-313

Specificity: This recombinant mouse monoclonal antibody recognizes a protein of 38 kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa or Y79 cells. Human pancreas, cerebellum or pheochromocytoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP

Background: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH,Synaptophysin (Neuroendocrine Marker)
Cellular localisation: Cytoplasm.

Molecular Weight: 38kDa

Gene ID: 6855, 632804

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity