MLX antibody

Catalog No. ABIN7126996

The Mouse Monoclonal anti-MLX antibody is suitable to detect MLX in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for MLX antibody (ABIN7126996)

Target: MLX (MAX-Like Protein X (MLX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MLX Antibody

Specificity: Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad1, Mad3, Mad4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad1, Mad4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MLX protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MLX

Target: MLX (MAX-Like Protein X (MLX))

Alternative Name: MLX

Background: BHLHd13, Class D basic helix-loop-helix protein 13, MAD7, Max-like bHLHZip protein, Max-like protein X, MLXIP, MONDOA, MXD7, Protein BigMax, Protein Mlx, Transcription factor-like protein 4 (TCFL4),MLX
Cellular localisation: Nucleus and cytoplasm.

Molecular Weight: 30kDa

Gene ID: 6945

UniProt: Q9UH92