Recombinant Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN7127010

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1352-1493) (ABIN7127010)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1352-1493

Specificity: It recognizes a 170 kDa protein, which is identified as topoisomerase IIi±. It shows no cross-reaction with Topoisomerase II i² or I. Topo II i± plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II i± is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Cross-Reactivity (Details): Human and Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A,Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Cellular localisation: Nuclear

Molecular Weight: 170kDa

Gene ID: 7153, 156346

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases