GTF2H2C antibody

Catalog No. ABIN7127037

This Mouse Monoclonal antibody specifically detects GTF2H2C in WB, IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for GTF2H2C antibody (ABIN7127037)

Target: GTF2H2C (General Transcription Factor IIH, Polypeptide 2C (GTF2H2C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GTF2H2C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-GTF2H2C Antibody

Specificity: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Cross-Reactivity (Details): Human. Predicted to react in Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GTF2H2C protein

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Raji cells. Human breast or prostate carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GTF2H2C

Target: GTF2H2C (General Transcription Factor IIH, Polypeptide 2C (GTF2H2C))

Alternative Name: GTF2H2C

Background: EVR6, PFM14, PR domain zinc finger protein 17 (PRDM17), RP72, Zinc finger protein 408 (GTF2H2C),GTF2H2C (Transcription Factor)
Cellular localisation: Nucleus. Nuclear speckles.

Molecular Weight: 44kDa

Gene ID: 730394, 422901

UniProt: Q6P1K8