TIM3 antibody (AA 22-202)

Catalog No. ABIN7127103

This Mouse Monoclonal antibody specifically detects TIM3 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for TIM3 antibody (AA 22-202) (ABIN7127103)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TIM3-4028

Product Details anti-TIM3 Antibody

Binding Specificity: AA 22-202

Purpose: TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker) Antibody

Specificity: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cel tonsil, lymph node or spleen. ls (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 or Daudi cells. Human tonsil, lymph node or spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: HAVCR2

Target Type: Virus

Background:

CD366, HAVR2, Hepatitis A virus cellular receptor 2 (HAVCR2), Kidney injury molecule 3 (KIM3), T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM3, TIMD3

HepG2 or Daudi cells. Human Tonsil, lymph node or spleen.

Molecular Weight: 60kDa

Gene ID: 84868, 710500

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints