CBFB antibody

Catalog No. ABIN7127112

This Mouse Monoclonal antibody specifically detects CBFB in WB, ELISA, IF, IP, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for CBFB antibody (ABIN7127112)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-CBFB Antibody

Specificity: The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, i± and i², which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2i± subunits, PEBP2i±A, PEBP2i±B and PEBP2i±C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory i² subunit, which facilitates DNA binding of the i± subunit. Both subunits are required for in vivo function, the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2i² is the target of chromosomal inversion 16 (p13,q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2i²/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CBFB protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFB

Background: CBF b; CBFB; Core-binding factor, beta subunit (CBFB), transcript variant 2; PEBP2 beta; PEBP2B; Polyomavirus enhancer binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit,CBFB / PEBP2β
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molecular Weight: 22kDa

Gene ID: 865, 519632

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha