CBFB antibody

Catalog No. ABIN7127113

The Mouse Monoclonal anti-CBFB antibody is suitable to detect CBFB in samples from Human. It has been validated for WB, ELISA, IF, IP, FACS and Coat.

Quick Overview for CBFB antibody (ABIN7127113)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-CBFB Antibody

Specificity: The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, i± and i², which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2i± subunits, PEBP2i±A, PEBP2i±B and PEBP2i±C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory i² subunit, which facilitates DNA binding of the i± subunit. Both subunits are required for in vivo function, the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2i² is the target of chromosomal inversion 16 (p13,q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2i²/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CBFB protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFB

Background: CBF b; CBFB; Core-binding factor, beta subunit (CBFB), transcript variant 2; PEBP2 beta; PEBP2B; Polyomavirus enhancer binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit,CBFB / PEBP2β
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molecular Weight: 22kDa

Gene ID: 865, 519632

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha