TRIM24 antibody

Catalog No. ABIN7127116

The Mouse Monoclonal anti-TRIM24 antibody has been validated for IF and FACS. It is suitable to detect TRIM24 in samples from Human.

Quick Overview for TRIM24 antibody (ABIN7127116)

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM24 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TRIM24 Antibody

Specificity: TIF1i±, also known as TRIM24, mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. TIF1i± localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. TIF1i± is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. The TIF1i± gene, which maps to human chromosome 7q33, encodes two alternatively spliced transcripts. However, the full length nature of one variant has not been determined. A TIF1i± homolog (designated bonus) has been identified in Drosophila and is associated with several genes that are implicated in the ecdysone pathway, a nuclear hormone receptor pathway required throughout Drosophila development, suggesting a conserved functional role for the protein throughout the course of evolution.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human TRIM24 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TRIM24

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Alternative Name: TRIM24

Background: E3 ubiquitin protein ligase TRIM24, hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1 alpha, Transcriptional intermediary factor 1 alpha, Tripartite motif-containing protein 24,TRIM24 / TIF1 alpha
Cellular localisation: Nucleus. Cytoplasm. Colocalizes with sites of active transcription.

Molecular Weight: 117kDa

Gene ID: 8805, 490287

UniProt: O15164