Recombinant CD5 antibody (AA 450-495)

Catalog No. ABIN7127139

This Mouse Monoclonal antibody specifically detects CD5 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD5 antibody (AA 450-495) (ABIN7127139)

Target: CD5

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RC5-6462

Product Details anti-CD5 Antibody

Binding Specificity: AA 450-495

Purpose: Recombinant CD5 (Mantle Cell Lymphoma Marker) Antibody

Specificity: The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10 % of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20 % of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa450-495) of human CD5 protein.

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Ramos, or MOLT-4 cells. Human tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background:

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

293T, or MOLT-4 cells. Human tonsil or lymph node., Ramos, K-562.

Molecular Weight: ~67kDa

Gene ID: 921, 58685

UniProt: P06127