Recombinant CD14 antibody

Catalog No. ABIN7127160

This Rabbit Monoclonal antibody specifically detects CD14 in ISt and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant CD14 antibody (ABIN7127160)

Target: CD14

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD14 Antibody

Specificity: CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans' cells, follicular dendritic reticulum cells, neutrophils (weakly), and endothelial cells of the liver sinusoids.CD14 is detected in myeloid leukemias with monoblastic/monocytic differentiation, and in histiocytic neoplasms like histiocytic lymphoma, Langerhans' cell histiocytosis and giant cell tumor. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues withinaa1-100 of CD14 was used as an immunogen.

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Lipopolysaccharide receptor, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein,CD14 (Monocyte / Macrophage Marker)
Cellular localisation: Cell Surface

Molecular Weight: 40kDa

Gene ID: 929, 163867

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades