Recombinant CD163 antibody (AA 1056-1156)

Catalog No. ABIN7127171

The Rabbit Monoclonal anti-CD163 antibody (Clone M130-3708R) (ABIN7127171) specifically detects CD163 in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD163 antibody (AA 1056-1156) (ABIN7127171)

Target: CD163

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: M130-3708R

Product Details anti-CD163 Antibody

Binding Specificity: AA 1056-1156

Purpose: Recombinant CD163 (Monocyte & Macrophage Marker) Antibody

Specificity: CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to CD163 residues within aa1056-1156 of CD163 was used as an immunogen

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: hPBL cells. Skin, lung, placenta or histiocytoma tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background:

Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130

HPBL cells. Skin, Lung, placenta or histiocytoma tissue.

Molecular Weight: 140kDa

Gene ID: 9332, 504641

UniProt: Q86VB7