Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MED21 antibody

MED21 Reactivity: Human WB, ELISA, IF, Coat, FACS, IP Host: Mouse Monoclonal unconjugated
Catalog No. ABIN7127181
  • Target See all MED21 Antibodies
    MED21 (Mediator Complex Subunit 21 (MED21))
    Reactivity
    • 23
    • 16
    • 6
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 18
    • 5
    Mouse
    Clonality
    • 19
    • 4
    Monoclonal
    Conjugate
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MED21 antibody is un-conjugated
    Application
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)
    Specificity
    In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity
    Cross-Reactivity (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Recombinant full-length human MED21 protein
    Isotype
    IgG1
    Top Product
    Discover our top product MED21 Primary Antibody
  • Application Notes

    Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or K562 cells.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Preservative
    Azide free
    Storage
    -20 °C,-80 °C
    Storage Comment
    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Expiry Date
    24 months
  • Target
    MED21 (Mediator Complex Subunit 21 (MED21))
    Alternative Name
    MED21 (MED21 Products)
    Synonyms
    SURB7 antibody, fk48d02 antibody, surb7 antibody, wu:fk48d02 antibody, zgc:56532 antibody, SRB7 antibody, hSrb7 antibody, 0610007L03Rik antibody, AI449604 antibody, D19234 antibody, D6Ertd782e antibody, Srb7 antibody, Surb7 antibody, T4B21.8 antibody, T4B21_8 antibody, mediator 21 antibody, mediator complex subunit 21 antibody, Med21p antibody, mediator 21 antibody, MED21 antibody, med21 antibody, Med21 antibody
    Background
    Forkhead box protein Q1, MED21, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH1, HNF3/forkhead like protein 1, MGC77469, Q1, Winged helix / forkhead transcription factor, zgc:77469,MED21 / SRB7
    Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.
    Molecular Weight
    15.5kDa
    Gene ID
    9412, 286145
    UniProt
    Q13503
    Pathways
    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
You are here:
Support