Recombinant NAPSA antibody (AA 1-100)

Catalog No. ABIN7127197

The Mouse Monoclonal anti-NAPSA antibody is suitable to detect NAPSA in samples from Human. It has been validated for IHC and WB.

Quick Overview for Recombinant NAPSA antibody (AA 1-100) (ABIN7127197)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPSA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RNAPSA-6926

Product Details anti-NAPSA Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody

Specificity: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant human Napsin-A protein fragment (aa1-100) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Lung adenocarcinoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: NAPSA

Background:

ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Human lung adenocarcinoma, Human Kidney

Molecular Weight: 37kDa

Gene ID: 9476, 512843

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis