RBM8A antibody

Catalog No. ABIN7127238

This Mouse Monoclonal antibody specifically detects RBM8A in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for RBM8A antibody (ABIN7127238)

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBM8A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-RBM8A Antibody

Specificity: Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human RBM8A protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RBM8A

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Alternative Name: RBM8A

Background: Binder of OVCA1-1, BOV1A, BOV1B, BOV1C, HSPC114, MDS014, ZRNP1, Ribonucleoprotein RBM8A, RNA-binding protein 8A, RNA-binding protein Y14,Y14 / RBM8A
Cellular localisation: Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.

Molecular Weight: 24kDa

Gene ID: 9939, 728824

UniProt: Q9Y5S9