KRT10, KRT13 antibody

Catalog No. ABIN7127244

This Mouse Monoclonal antibody specifically detects KRT10, KRT13 in FACS, IF, IHC and WB. It exhibits reactivity toward Cat and Human.

Quick Overview for KRT10, KRT13 antibody (ABIN7127244)

Target: KRT10, KRT13

Reactivity: Cat, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: DE-K13

Product Details

Purpose: Cytokeratin 10/13 Antibody

Specificity: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Cross-Reactivity (Details): Human and Cat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: A431, HeLa or MCF7 cells. Human tonsil, esophageal or bladder carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT10, KRT13

Alternative Name: KRT10 & KRT13

Background:

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT13, Type I keratin Ka13

A431, esophageal or bladder carcinoma., HeLa or MCF7 cells. Human tonsil

Molecular Weight: 56.5kDa (CK10) & 53kDa (CK13)