Recombinant KRT antibody

Catalog No. ABIN7127255

The Mouse Monoclonal anti-KRT antibody (Clone Cocktail rPCK-6750) (ABIN7127255) specifically detects KRT in WB, IHC, FACS and IF. The antibody is reactive with Human, Cat and Dog samples.

Quick Overview for Recombinant KRT antibody (ABIN7127255)

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Antibody Type: Recombinant Antibody

Reactivity: Human, Cat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: Cocktail rPCK-6750

Product Details anti-KRT Antibody

Purpose: Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

Specificity: Anti-cytokeratin clone rPCK/6750 (CK rPCK/6750) demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, rPCK/6750 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, rPCK/6750 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Cross-Reactivity (Details): Human, Dog and Cat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Skin, adeno- or squamous carcinomas (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Alternative Name: KRT

Background:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

adeno- or squamous carcinomas (IHC)., Skin, A431.

Molecular Weight: 40-67kDa ()