Recombinant Cytokeratin 2 antibody (AA 300-400)

Catalog No. ABIN7127260

The Rabbit Monoclonal anti-Cytokeratin 2 antibody has been validated for IHC (f). It is suitable to detect Cytokeratin 2 in samples from Human.

Quick Overview for Recombinant Cytokeratin 2 antibody (AA 300-400) (ABIN7127260)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Cytokeratin 2 Antibody

Binding Specificity: AA 300-400

Specificity: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Alternative Name: KRT76

Background: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Type II (Epithelial Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 66/65/64/57/62/60/51/54kDa

Gene ID: 51350, 654392

UniProt: Q01546