Recombinant ARNT antibody

Catalog No. ABIN7127344

The Rabbit Monoclonal anti-ARNT antibody (Clone 2F11) (ABIN7127344) specifically detects ARNT in WB, IHC, ELISA and IP. The antibody is reactive with Human samples.

Quick Overview for Recombinant ARNT antibody (ABIN7127344)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: 2F11

Product Details anti-ARNT Antibody

Purpose: ARNT Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human ARNT

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT

Background:

Background: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).

Aliases: Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect