Recombinant BRD4 antibody

Catalog No. ABIN7127371

This anti-BRD4 antibody is a Rabbit Monoclonal antibody detecting BRD4 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for Recombinant BRD4 antibody (ABIN7127371)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1E5

Product Details anti-BRD4 Antibody

Cross-Reactivity: Human

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Brd4

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background:

Background: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

Aliases: Bromodomain-containing protein 4 (Protein HUNK1), BRD4, HUNK1

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome