Recombinant ITK antibody

Catalog No. ABIN7127585

This Rabbit Monoclonal antibody specifically detects ITK in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant ITK antibody (ABIN7127585)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ITK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: 8F10

Product Details anti-ITK Antibody

Cross-Reactivity: Human

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human ITK

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background:

Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

Aliases: Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2) (Interleukin-2-inducible T-cell kinase) (IL-2-inducible T-cell kinase) (Kinase EMT) (T-cell-specific kinase) (Tyrosine-protein kinase Lyk), ITK, EMT LYK

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway