Recombinant YAP1 antibody (pSer127)

Catalog No. ABIN7127761

The Rabbit Monoclonal anti-YAP1 antibody (Clone 3F3) (ABIN7127761) specifically detects YAP1 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant YAP1 antibody (pSer127) (ABIN7127761)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3F3

Product Details anti-YAP1 Antibody

Binding Specificity: pSer127

Purpose: Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-YAP1 (S127)

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5000, IHC:1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background:

Background: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).

Aliases: Transcriptional coactivator YAP1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, YAP1, YAP65

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha