Recombinant PKM antibody

Catalog No. ABIN7127769

The Rabbit Monoclonal anti-PKM antibody has been validated for ELISA, WB, IHC, IF, IP and FACS. It is suitable to detect PKM in samples from Human.

Quick Overview for Recombinant PKM antibody (ABIN7127769)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 7B2

Product Details anti-PKM Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PKM2

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200, IP:1:200-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background:

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Aliases: Pyruvate kinase PKM (EC 2.7.1.40) (Cytosolic thyroid hormone-binding protein) (CTHBP) (Opa-interacting protein 3) (OIP-3) (Pyruvate kinase 2/3) (Pyruvate kinase muscle isozyme) (Thyroid hormone-binding protein 1) (THBP1) (Tumor M2-PK) (p58), PKM, OIP3 PK2 PK3 PKM2

UniProt: P14618

Pathways: Warburg Effect