ACOT8 antibody (AA 31-130) (HRP)

Catalog No. ABIN708650

The Rabbit Polyclonal anti-ACOT8 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect ACOT8 in samples from Human.

Quick Overview for ACOT8 antibody (AA 31-130) (HRP) (ABIN708650)

Target: ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACOT8 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ACOT8 Antibody (HRP)

Binding Specificity: AA 31-130

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ACOT8

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ACOT8

Target: ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))

Alternative Name: ACOT8

Background:

Synonyms: ACTEIII, Acot8, ACOT8_HUMAN, acyl CoA thioesterase 8, Acyl-CoA thioesterase 8, Acyl-coenzyme A thioesterase 8, Choloyl coenzyme A thioesterase, Choloyl-coenzyme A thioesterase, hACTE III, hACTE-III, hACTEIII, HIV Nef associated acyl CoA thioesterase, HIV-Nef-associated acyl-CoA thioesterase, HNAACTE, hTE, Long chain fatty acyl CoA hydrolase, Palmitoyl CoA hydrolase, Peroxisomal acyl CoA thioesterase 1, Peroxisomal acyl coenzyme A thioester hydrolase 1, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long chain acyl CoA thioesterase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, PTE 1, PTE 2, PTE-1, PTE-2, PTE1, PTE2, Thioesterase II.

Background: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A Thioesterase 8, also known as ACOT8 may mediate Nef-induced down-regulation of CD4. It is a major thioesterase in peroxisomes and competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). It shows a preference for medium-length fatty acyl-CoAs and may be involved in the metabolic regulation of peroxisome proliferation.

Gene ID: 10005

Pathways: Monocarboxylic Acid Catabolic Process