HMGCL antibody (AA 41-140) (Cy3)

Catalog No. ABIN709184

The Rabbit Polyclonal anti-HMGCL antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect HMGCL in samples from Mouse.

Quick Overview for HMGCL antibody (AA 41-140) (Cy3) (ABIN709184)

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCL antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-HMGCL Antibody (Cy3)

Binding Specificity: AA 41-140

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HMGCL

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HMGCL

Target: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Alternative Name: HMGCL

Background:

Synonyms: 3 hydroxy 3 methylglutaryl CoA lyase, 3 hydroxy 3 methylglutaryl Coenzyme A lyase, 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase hydroxymethylglutaricaciduria, 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HL, HMG CoA lyase, HMG CoA Lyase Deficiency, HMG-CoA lyase, HMGCL, HMGCL_HUMAN, Hydroxymethylglutaricaciduria, Hydroxymethylglutaryl CoA lyase, Hydroxymethylglutaryl CoA lyase mitochondrial, Hydroxymethylglutaryl-CoA lyase, mitochondrial, MS725, OTTHUMP00000044830.

Background: Hydroxymethylglutaryl-CoA lyase (HMGCL) is found in fibroblasts, liver and lymphoblasts. It has a role in ketogenesis and leucine catabolism. Defects in HMGCL are the cause of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (hydroxymethylglutaricaciduria), an autosomal recessive disease which can lead to hypoglycemia and coma.

Gene ID: 2835