ME1 antibody (AA 451-550)

Catalog No. ABIN709436

The Rabbit Polyclonal anti-ME1 antibody has been validated for WB, ELISA, IHC (p), IHC (fro), IF (cc) and IF (p). It is suitable to detect ME1 in samples from Rat.

Quick Overview for ME1 antibody (AA 451-550) (ABIN709436)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ME1 Antibody

Binding Specificity: AA 451-550

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Malic enzyme 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1

Background:

Synonyms: Cytosolic malic enzyme 1, HUMNDME, Malate dehydrogenase, Malic enzyme 1, Malic enzyme 1, NADP+ dependent, cytosolic, Malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP dependent malic enzyme, NADP ME, Pyruvic malic carboxylase.Cytosolic malic enzyme 1, HUMNDME, Malate dehydrogenase, Malic enzyme 1, Malic enzyme 1, NADP+ dependent, cytosolic, Malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP dependent malic enzyme, NADP ME, Pyruvic malic carboxylase.

Background: ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Gene ID: 4199

Pathways: Regulation of Lipid Metabolism by PPARalpha