TWIST1 antibody

Catalog No. ABIN7138095

The Rabbit Polyclonal anti-TWIST1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TWIST1 in samples from Human.

Quick Overview for TWIST1 antibody (ABIN7138095)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TWIST1 Antibody

Purpose: TWIST1 Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Peptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1

Background:

Background:

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.Cell 112:169-180(2003) 
"Mutations of the TWIST gene in the Saethre-Chotzen syndrome."el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46(1997)

Aliases: ACS3 antibody; B-HLH DNA binding protein antibody; bHLHa38 antibody; BPES2 antibody; BPES3 antibody; Class A basic helix-loop-helix protein 38 antibody; CRS antibody; CRS1 antibody; CSO antibody; H-twist antibody; OTTHUMP00000116043 antibody; SCS antibody; Twist basic helix loop helix transcription factor 1 antibody; Twist family bHLH transcription factor 1 antibody; Twist homolog 1 (Drosophila) antibody; Twist homolog 1 antibody; TWIST homolog of drosophila antibody; Twist related protein 1 antibody; Twist-related protein 1 antibody; TWIST1 antibody; TWST1_HUMAN antibody

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity