MYOD1 antibody (pSer200)

Catalog No. ABIN7138470

The Rabbit Polyclonal anti-MYOD1 antibody has been validated for WB and ELISA. It is suitable to detect MYOD1 in samples from Human.

Quick Overview for MYOD1 antibody (pSer200) (ABIN7138470)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MYOD1 Antibody

Binding Specificity: pSer200

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD1

Background:

Background: MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Emmanuel G, et al. J. Biol. Chem., Jun 2000, 275: 18767 - 18776
Tintignac LA, et al. Mol Cell Biol. 2004 Feb, 24(4): 1809-1821.
Kitzmann M, et a. Mol Cell Biol. 1999 Apr, 19(4): 3167-3176.

Aliases: bHLHc1 antibody, Class C basic helix-loop-helix protein 1 antibody, MYF 3 antibody, Myf-3 antibody, MYF3 antibody, Myoblast determination protein 1 antibody, Myod 1 antibody, MYOD antibody, MYOD1 antibody, MYOD1_HUMAN antibody, Myogenic differentiation 1 antibody, Myogenic factor 3 antibody, Myogenic factor MYF 3 antibody, Myogenin D1 antibody, PUM antibody

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development