G3BP1 antibody (pSer232)

Catalog No. ABIN7138496

This Rabbit Polyclonal antibody specifically detects G3BP1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human.

Quick Overview for G3BP1 antibody (pSer232) (ABIN7138496)

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-G3BP1 Antibody

Binding Specificity: pSer232

Purpose: Phospho-G3BP1 (Ser232) Antibody

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP1

Background:

Background: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

Aliases: ATP dependent DNA helicase VIII antibody, ATP-dependent DNA helicase VIII antibody, G3BP antibody, G3BP stress granule assembly factor 1 antibody, G3BP-1 antibody, G3bp1 antibody, G3BP1_HUMAN antibody, GAP binding protein antibody, GAP SH3 domain binding protein 1 antibody, GAP SH3 domain-binding protein 1 antibody, GTPase activating protein (SH3 domain) binding protein 1 antibody, hDH VIII antibody, Human DNA helicase VIII antibody, MGC111040 antibody, Ras GTPase activating protein binding protein 1 antibody, Ras GTPase activating protein SH3 domain binding protein antibody, Ras GTPase-activating protein-binding protein 1 antibody, RasGAP associated endoribonuclease G3BP antibody

UniProt: Q13283

Pathways: SARS-CoV-2 Protein Interactome