PDPK1 antibody (pSer241)

Catalog No. ABIN7138502

This Rabbit Polyclonal antibody specifically detects PDPK1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for PDPK1 antibody (pSer241) (ABIN7138502)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PDPK1 Antibody

Binding Specificity: pSer241

Purpose: Phospho-PDPK1 (Ser241) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1

Background:

Background: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Scheid MP,et al. (2005)Mol Cell Biol, 25(6): 2347-63
Chen H, et al. (2001) Biochemistry, 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem, 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A, 100(24): 14006-11

Aliases: 3 phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, hPDK 1 antibody, hPDK1 antibody, MGC20087 antibody, MGC35290 antibody, OTTHUMP00000159109 antibody, OTTHUMP00000159110 antibody, OTTHUMP00000174525 antibody, PDK1 antibody, Pdpk1 antibody, PDPK1_HUMAN antibody, PDPK2 antibody, PDPK2P antibody, PkB kinase antibody, PkB kinase like gene 1 antibody, PkB like 1 antibody, PRO0461 antibody, Protein kinase antibody

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals