PDPK1 antibody (pSer241)

Catalog No. ABIN7138502

Product Details anti-PDPK1 Antibody

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Binding Specificity: pSer241

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1 (PDPK1 Products)

Background:

Background: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Scheid MP,et al. (2005)Mol Cell Biol, 25(6): 2347-63
Chen H, et al. (2001) Biochemistry, 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem, 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A, 100(24): 14006-11

Aliases: 3 phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, hPDK 1 antibody, hPDK1 antibody, MGC20087 antibody, MGC35290 antibody, OTTHUMP00000159109 antibody, OTTHUMP00000159110 antibody, OTTHUMP00000174525 antibody, PDK1 antibody, Pdpk1 antibody, PDPK1_HUMAN antibody, PDPK2 antibody, PDPK2P antibody, PkB kinase antibody, PkB kinase like gene 1 antibody, PkB like 1 antibody, PRO0461 antibody, Protein kinase antibody

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals