HSF1 antibody (pSer307)

Catalog No. ABIN7138540

This anti-HSF1 antibody is a Rabbit Polyclonal antibody detecting HSF1 in WB, ELISA and IF. Suitable for Human.

Quick Overview for HSF1 antibody (pSer307) (ABIN7138540)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-HSF1 Antibody

Binding Specificity: pSer307

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Background:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Guettouche T, et al. (2005) BMC Biochem , 6(1): 4
Wang X, et al. (2003) Mol Cell Biol , 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA, 96(12): 6769-6774.

Aliases: Heat shock factor 1 antibody, Heat shock factor protein 1 antibody, Heat shock transcription factor 1 antibody, HSF 1 antibody, hsf1 antibody, HSF1_HUMAN antibody, HSTF 1 antibody, HSTF1 antibody

UniProt: Q00613