ENOS antibody (pSer615)

Catalog No. ABIN7138659

The Rabbit Polyclonal anti-ENOS antibody is suitable to detect ENOS in samples from Human. It has been validated for WB and ELISA.

Quick Overview for ENOS antibody (pSer615) (ABIN7138659)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ENOS Antibody

Binding Specificity: pSer615

Purpose: Phospho-NOS3 (Ser615) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 615 (F-N-S(p)-I-S) derived from Human eNOS.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background:

Background: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Aliases: cNOS antibody, Constitutive NOS antibody, EC NOS antibody, EC-NOS antibody, ecNOS antibody, Endothelial nitric oxidase synthase antibody, Endothelial nitric oxide synthase antibody, Endothelial nitric oxide synthase 3 antibody, Endothelial NOS antibody, eNOS antibody, Nitric oxide synthase 3 (endothelial cell) antibody, Nitric oxide synthase 3 antibody, Nitric oxide synthase 3 endothelial cell antibody, Nitric oxide synthase endothelial antibody, Nitric oxide synthase, endothelial antibody, NOS 3 antibody, NOS III antibody, NOS type III antibody, NOS3 antibody, NOS3_HUMAN antibody, NOSIII antibody

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling