KRT8 antibody (pSer74)

Catalog No. ABIN7138693

The Rabbit Polyclonal anti-KRT8 antibody (ABIN7138693) specifically detects KRT8 in WB, IF and ELISA. The antibody is reactive with Human samples.

Quick Overview for KRT8 antibody (pSer74) (ABIN7138693)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-KRT8 Antibody

Binding Specificity: pSer74

Purpose: Phospho-KRT8 (Ser74) Antibody

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: KRT8

Background:

Background:

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Nakamichi I, et al. (2002) Mol Biol Cell, 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res, 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A, 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol, 13(11): 6742-6751

Aliases: CARD2 antibody, CK 8 antibody, CK-8 antibody, CK8 antibody, CYK8 antibody, CYKER antibody, Cytokeratin endo A antibody, Cytokeratin-8 antibody, DreK8 antibody, EndoA antibody, K2C8 antibody, K2C8_HUMAN antibody, K8 antibody, Keratin 8 antibody, Keratin type II cytoskeletal 8 antibody, Keratin, type II cytoskeletal 8 antibody, Keratin-8 antibody, KO antibody, Krt 2.8 antibody, KRT8 antibody, MGC118110 antibody, MGC174782 antibody, MGC53564 antibody, MGC85764 antibody, sb:cb186 antibody, Type-II keratin Kb8 antibody

UniProt: P05787