Src antibody (pSer75)

Catalog No. ABIN7138694

This Rabbit Polyclonal antibody specifically detects Src in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Src antibody (pSer75) (ABIN7138694)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Src Antibody

Binding Specificity: pSer75

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 75 (V-T-S(p)-P-Q) derived from Human Src.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC

Background:

Background:

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme, phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pan Q, et al. (2011)Cell Mol Life Sci 68, 3425-36 . Kato G, Maeda S (2003) J Biochem (Tokyo) 133, 563-9. Kato G, Maeda S (1999)J Biochem (Tokyo) 126, 957-61.

Aliases: ASV antibody, Avian sarcoma virus antibody, AW259666 antibody, c SRC antibody, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody, cSrc antibody, EC 2.7.10.2 antibody, Neuronal CSRC tyrosine specific protein kinase antibody, Neuronal proto-oncogene tyrosine-protein kinase Src antibody, Neuronal SRC antibody, Oncogene SRC antibody, OTTHUMP00000174476 antibody, OTTHUMP00000174477 antibody, p60 Src antibody, p60-Src antibody, p60c-src antibody, p60Src antibody, pp60c src antibody, pp60c-src antibody, pp60csrc antibody, Proto oncogene tyrosine protein kinase Src antibody, Proto-oncogene c-Src antibody, Proto-oncogene tyrosine-protein kinase Src antibody, Protooncogene SRC antibody, Protooncogene SRC Rous sarcoma antibody, Src antibody, SRC Oncogene antibody, SRC proto oncogene non receptor tyrosine kinase antibody, SRC_HUMAN antibody, SRC1 antibody, Tyrosine kinase pp60c src antibody, Tyrosine protein kinase SRC 1 antibody, Tyrosine protein kinase SRC1 antibody, v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody, V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody, v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling