CDC25A antibody (pSer76)

Catalog No. ABIN7138695

The Rabbit Polyclonal anti-CDC25A antibody has been validated for WB, ELISA and IHC. It is suitable to detect CDC25A in samples from Human.

Quick Overview for CDC25A antibody (pSer76) (ABIN7138695)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CDC25A Antibody

Binding Specificity: pSer76

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background:

Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

Aliases: Cdc 25a antibody, CDC25A antibody, CDC25A2 antibody, CDC25A2 CAG isoform antibody, Cell division cycle 25 homolog A (S. pombe) antibody, Cell division cycle 25A antibody, Cell division cycle 25A isoform a antibody, Cell division cycle 25A isoform b antibody, D9Ertd393e antibody, Dual specificity phosphatase Cdc25A antibody, M phase inducer phosphatase 1 antibody, M-phase inducer phosphatase 1 antibody, MGC115549 antibody, MPIP1_HUMAN antibody

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase