ZAP70 antibody (pTyr319)

Catalog No. ABIN7138978

This anti-ZAP70 antibody is a Rabbit Polyclonal antibody detecting ZAP70 in WB, IHC, ELISA and IF. Suitable for Human.

Quick Overview for ZAP70 antibody (pTyr319) (ABIN7138978)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-ZAP70 Antibody

Binding Specificity: pTyr319

Purpose: Phospho-ZAP70 (Tyr319) Antibody

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background:

Background:

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Salomon AR, et al. (2003) Proc Natl Acad Sci U S A, 100(2): 443-448
Ku GM, et al. (2001) EMBO J, 20(3): 457-465
Tang J, et al. (1999) Proc Natl Acad Sci U S A, 96(17): 9775-9780
Zhao Q, et al. (1996) Mol Cell Biol, 16(12): 6765-6774
Williams BL, et al. (1999) EMBO J, 18(7): 1832-1844
Adjali O, et al. (2005) J Clin Invest, 115(8): 2287-2295.

Aliases: 70 kDa zeta associated protein antibody, 70 kDa zeta-associated protein antibody, EC 2.7.10.2 antibody, FLJ17670 antibody, FLJ17679 antibody, Selective T cell defect antibody, SRK antibody, STD antibody, Syk related tyrosine kinase antibody, Syk-related tyrosine kinase antibody, Truncated ZAP kinase antibody, Tyrosine protein kinase ZAP70 antibody, Tyrosine-protein kinase ZAP-70 antibody, TZK antibody, ZAP 70 antibody, ZAP70 antibody, ZAP70_HUMAN antibody, Zeta chain associated protein kinase 70kD antibody, Zeta chain associated protein kinase 70 kDa antibody, Zeta chain associated protein kinase 70 kDa isoform 1 antibody, Zeta chain associated protein kinase 70 kDa isoform 2 antibody, Zeta chain of T cell receptor associated protein kinase 70 antibody, Zeta chain TCR associated protein kinase 70kD antibody, Zeta chain TCR associated protein kinase 70 kDa antibody

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway