HCK antibody (pTyr521)

Catalog No. ABIN7139019

The Rabbit Polyclonal anti-HCK antibody has been validated for WB and ELISA. It is suitable to detect HCK in samples from Human.

Quick Overview for HCK antibody (pTyr521) (ABIN7139019)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HCK Antibody

Binding Specificity: pTyr521

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 521(E-S-Q(p)-Y-Q) derived from Human HCK .

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: HCK

Background:

Background:

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Quintrell N., Mol. Cell. Biol. 7:2267-2275(1987) [PubMed: 3496523].
Ziegler S.F.,Mol. Cell. Biol. 7:2276-2285(1987) [PubMed: 3453117].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].

Aliases: Bmk antibody, Hck 1 antibody, Hck antibody, HCK_HUMAN antibody, Hemopoietic cell kinase antibody, JTK9 antibody, p59-HCK/p60-HCK antibody, p59Hck antibody, p59HCK/p60HCK antibody, p61Hck antibody, Tyrosine protein kinase HCK antibody, Tyrosine-protein kinase HCK antibody

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling